"Arabidopsis were sown in rows inside glass jars (15 seeds per 9-cm diameter jar) containing SILIA agar medium (either with K 14 NO3 or K 15 NO 3 ) and grown in growth chamber as described previously .Both 14 N- and 15 N-labelled 3-week-old flowering plants were treated in pairs. 15 N-coded plants were subjected to a hydrogen peroxide treatment and the 14 N-labelled plants were used as the control (i.e. forward labelling) or vice versa (i.e. reciprocal labelling).
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Citation: Hu Q, Guo G, Yang Z, Li Y, Xia Y (2014) Stable Isotope Metabolic Labeling-Based Quantitative Thiol Redox Proteomic Analysis of Hydrogen Peroxide-treated Arabidopsis plant. J Proteomics Bioinform 7: 121-133."
Last date updated on July, 2014