|"The assimilation of NH4+ in the fungal cell for amino acid synthesis could reduce the cytosolic pH because NH4+ is converted to NH3 and the excess H+ is introduced into the cytoplasm. This H+ is released into the external solution to maintain cytoplasmic pH, acidifying the medium surrounding the fungal cells and thereby favoring RP dissolution. Although NH4+ is a nutrient for fungi, it can become toxic at high concentrations. One problem due to an excessive level of NH4+ is its interference with the uptake of other fungal nutrients (Mg2+, Na +, Ca2+,etc.) as clearly seen in plants. However, the most negative effect of NH 4 is probably its interference with the electrochemical gradient that must be maintained between the cytoplasm and the external medium.
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