|Folliculogenesis is a processofovarian follicles growth and development, which is the interaction of oocyte and granulosa cells with resulting in mature and fertilizable oocytes. There are two crucial growth factors that play a role in the interaction of oocyte and granulosa cells, namely Growth Differentiation Factor-9 (GDF-9) derived from oocyte and Kit-Ligand produced from granulosa cells. In abnormal folliculogenesis, the interaction of both growth factors could disturb the growth of follicles in the ovaries. The use of bone marrow mesenchymal stem cells, which have the ability to regenerate itself and differentiate into other tissue cells, has been tried for regeneration therapy of various diseases. The study conducted on this aimed to evaluate the effect of allogeneic bone marrow transplantation on the oocyte-granulosa cell interaction by analyzing the expression of GDF- 9 and Kit-Ligand and the description of follicular development by analyzing the number of primordial, primary, secondary, and graafian follicles in cisplatin-induced ovarian failure in rat.
In cisplatin-induced ovarian failure in rat, bone marrow transplantation may improve oocyte-granulosa cell interaction and follicular development. Further study is required to confirmation that result, with some improvement of methodology such as: using GFP to trace the BMT in cells, add sham group (PBS and BMT) as a control, perform ELISA to confirm enhanced GDF-9 secretion and exploration the optimal dose of BMT.
Hendi Hendarto, The Effect of Bone Marrow Transplantation on Oocyte-Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat
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