Cancer biomarker discovery started to use proteomics a decade ago, when a mass-spectrometric top-down approach was developed specifically for that purpose, a time-of-flight mass-spectrometry with matrix-assisted laser desorption ionization (MALDI-TOF). The method could be used for direct profiling of biological fluids, for example, plasma or urine. Usually, the sample is subjected to fast separation or desalting and applied to the metallic chip. In this approach, proteins and peptides are recorded in its intact form, but remain unidentified unless downstream experiments are performed. vs Sergei Moshkovskii, Proteomics for cancer biomarkers: From MALDI-TOF profiling to targeted proteogenomics.
Journal of Proteomics & Bioinformatics from OMICS Group published best research and peer-review articles in the field of biomarkers and its applications of proteomics in the discovery of novel biomarkers.
Last date updated on June, 2014