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Human Epidermal Growth Factor Receptor 2 (HER2) is overexpressed in breast cancers and is a key predictive biomarker and an effective therapeutic treatment target for breast cancer. The vast evidence from various studies indicates that patients with HER2-positive breast cancer have a poorer prognosis than patients with HER2-negative breast cancer, and it requires a extraordinary therapy. Therefore, identifying the overexpression of HER2 for breast cancer diagnosis and therapy is very important. In recent years, several methods for detecting HER2-positive cancer cells have been developed, which include genomic and proteomic assays, optical assays, fluorescence, scattering assays, direct cell imaging.
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