alexa Three - (-) Catechin-O-Rhamnosides from the Eastern Nigeria Mistletoe with Potent Immunostimulatory and Antioxidant Activities

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Three - (-) Catechin-O-Rhamnosides from the Eastern Nigeria Mistletoe with Potent Immunostimulatory and Antioxidant Activities

In an attempt to provide further convincing evidence for the variously reported immunomodulatory potentials of mistletoes, bioassay-guided fractionation of the eastern Nigeria mistletoe afforded three compounds: - (-) catechin- 7-O-rhamnoside (1), - (-) catechin-3-O- rhamnoside (2) and a 4′-methoxy-7-O-rhamnoside (3). Their effects on C57BL6 mice splenocytes proliferation and expression of CD69 molecule were determined using flow cytometry techniques and compared to Lipopolysaccharide (LPS; 10 μg/ml) and Concanavalin A (ConA; 2 μg/ml) as standards. The antioxidant study was by the DPPH model with ascorbic acid as standard. The compounds (1-3) at 100 μg/ml showed statistically significant (p<0.05) stimulatory activity with values of 91.49 ± 0.22%, 95.17 ± 0.01% and 94.23 ± 0.07% respectively compared to 2.65 ± 0.33% for the unstimulated control. However, the CD69 expression assay showed only moderate stimulation. Their measured antioxidant potentials (effective concentration; EC50) were high (≤ 55.42 ± 0.99 mg/ml) when compared to ascorbic acid (17.6 ± 1.78 mg/ml). Characterization of the compounds was achieved by chromatographic and spectroscopic methods

Citation: Ogechukwu OE, Ogoamaka OP, Kawamura A, Hassan A, Debbab A, et al. (2012) Three - (-) Catechin-O-Rhamnosides from the Eastern Nigeria Mistletoe with Potent Immunostimulatory and Antioxidant Activities. Biomolecul 1:102. doi: 10.4172/2167-7956.1000102

 
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