alexa Reuse of Cyclodextrin Glycosyltransferase through Immobilization

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Reuse of Cyclodextrin Glycosyltransferase through Immobilization

CGTase from Bacillus lehensis isolated from wastewater of a cassava flour mill in the state of São Paulo, Brazil was immobilized after being partially purified using precipitation with ammonium sulfate. The partially purified CGTase was immobilized by covalent binding on a magnetite support, which was silanized with 3-aminopropyltrimethoxysilane and activated with glutaraldehyde, resulting in a yield of 16.27% and final activity of 17.54% of the initial activity.

 

Citation: Blanco KC, dos Santos FJ, Bernardi NS, Júnior MJ, Monti R, et al. (2013) Reuse of Cyclodextrin Glycosyltransferase through Immobilization on Magnetic Carriers. Enz Eng 2:111.

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