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As the fly larva grows, it keeps the same number of cells, but needs to make much more gene product. The result is that the cells get much bigger and each chromosome divides hundreds of times, but all the strands stay attached to each other. The result is a massively thick polytene chromosome, which can easily be seen under the microscope. These are the magic markers that first put Drosophila in the spotlight. These chromosomes have a pattern of dark and light bands, like a bar code, which is unique for each section of the chromosome.
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