Australian sport was rocked by the revelations of the Australian Crime Commission’s report Organized Crime and Drugs in Sport. Although the primary focus of the report concerns the use of prohibited substances at elite level, the report also examined the possible effects of prohibited substance use at sub-elite levels. Little is known about the use of medications, dietary supplements, and alcohol and tobacco by Australian sub-elite athletes. This paper provides a ‘snapshot’ of use at a single sub-elite Australian Rules Football Club prior to the commencement of the 2012 South Australian football league season. Players were required to complete a medical questionnaire prior to the commencement of the 2012 SANFL season. As part of the questionnaire, players were provided with questions concerning the use of prescription medications, supplements, and alcohol and tobacco. Players that were included on the ‘Senior Training Squad’ were identified and their questionnaires used in the preparation of this article.