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Antibiotics
Antibiotics or antibacterials are a type of antimicrobial used specifically against bacteria, and are often used in medical treatment of bacterial infections.
Methods
Several methods have already been established to analyse veterinary antibiotics and steroid hormones in water [8-20]. However, few procedures exist for soil, which is a more complex matrix, because of the presence of numerous interfering substances and the necessity of breaking interactions between target compounds and soil.
The goal of this work was to compare Pressurised Liquid Extraction (PLE), which is usually used to extract drugs from soil, and a modified-QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. Selective Pressurised Liquid Extraction (SPLE) and Dispersive Solid Phase Extraction (dSPE) used after PLE and QuEChERS, respectively, were tested. These techniques permit a fast and simple purification step. SPE, which is frequently used, was also evaluated. To perform this comparison, both recoveries and matrix effects were compared and the analyses were performed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
SPLE and dSPE did not significantly decrease matrix effects. A tandem SPE using SAX and Strata-X cartridges offered the best efficiency. Regarding the comparison between PLE and QuEChERS, the modified-QuEChERS led to better recoveries for certain substances.
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