alexa IVA: accurate de novo assembly of RNA virus genomes

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IVA: accurate de novo assembly of RNA virus genomes

An accurate genome assembly from short read sequencing data is serious for downstream analysis, allowing investigation of variants within a sequenced population. However, assembling sequencing data from virus samples, especially RNA viruses, into a genome sequence is challenging due to the amalgamation of viral population diversity and exceptionally uneven read depth caused by amplification bias in the inevitable reverse transcription and PCR amplification procedure of present approaches.

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