The present research work was undertaken in the Department of Horticulture, Mahatma Phule Krishi Vidyapeeth, Rahuri to evaluate recently developed somatic hybrid derivatives of Lycopersicon esculentum and Lycopersicon peruvianum Mill tomato species for beta carotene content. The field experiment consisting of eight orange fruited and five traditional red fruited tomato cultivars during rabi season in randomized block design with three replications. The recommended package of practices was followed for better production of the crop. The data revealed that, among the orange fruited cultivars, the highest beta carotene content was noticed in cv. 34A-1-1(7.03 mg/100 g) followed by cv. 42B-1-1 (4.00 mg/100 g) and cv. 22-1-1 (3.85 mg/100 g). Among traditional red fruited cultivars, cv. Dhanshree recorded the highest beta carotene content i.e. 1.30 mg/100 g followed by cv. Pusa Ruby (0.90 mg/100 g) and lowest in cv. Bhagyashree (0.41 mg/100 g). The orange fruited cultivars recorded higher mean beta carotene content i.e. 3.80 mg/100 g than the traditional red fruited cultivars (0.80 mg/100 g).