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A rapid, simple and sensitive method for the determination of D-Penicillamine using sodium 1,2-naphthoquine- 4-sulfonate (NQS) has been developed. The method is based on the fact that a brown product can be formed by the reaction between D-Penicillamine and sodium NQS in a buffer solution of a pH 12.0. Beer’s law is obeyed in the range 10-30 μg/mL of D-Penicillamine at maximum wavelength of 425 nm. The linear regression equation of the calibration curve is A=0.1684+0.01624C (μg/mL), with a linear regression correlation coefficient of 0.997. The detection limit is 3.12 μg/mL and the recovery rate is in the range of 99.21-108.2%. The method has been successfully applied to the determination of D-Penicillamine in pharmaceutical formulation.
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