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Peptide bond formation is a nucleophilic substitution reaction of an amino group (nucleophile) at a carboxyl group involving a tetrahedral intermediate. Furthermore, the peptide coupling reaction must be performed under mild conditions, and preferably at room temperature. Activation of the carboxyl component is achieved by the introduction of electron accepting moieties [1]. Carboxyl components can be activated as acyl halides, acyl azides, acylimidazoles, anhydrides, esters etc. There are different ways of coupling reactive carboxyl derivatives with an amine
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