Flemingia strobilifera (Fabaceae) is an important medicinal plant, commonly known as Kamalu in Malayalam and Kusrunt in Hindi. It is distributed in the region of the tropical area of India. Root of the plant is used for various ailments such as insomnia, epilepsy, ulcer, inflammation and microbial infection. However, Pharmacognostical standardization of this important medicinal plant has not been reported. Present study aims to characterize botanical and phytochemical features of the root, which could be used for the standard testing protocols in the future. Botanical and phytochemical features of root of this plant were analyzed. These features would be of great help in authentication and standardization of the raw drug. The phytochemical tests revealed the presence of carbohydrates, flavonoids, phenol, tannin, terpenoids, saponin, coumarins, quinone and steroids in the root of this plant. The HPLTC fingerprint obtained would be useful in chemical standardization of the raw drug of Kamalu.