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Simultaneous saccharification of corn starch and fermentation to gluconic acid (SSF) was examined with Aspergillus niger immobilized on nonwoven fabric under high dissolved oxygen (DO). The starch liquefaction step to maltodextrins by the action of thermostable α-amylase was optimized using the dextrose equivalent value as an index. SSF was carried out using liquefied starch with simultaneous addition of commercial amyloglucosidase, and A. niger for gluconic acid production at DO of 150 mg/l by supplying pressurized oxygen gas.
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