Absolutely the largest proportion of the Earth’s biosphere is consisting of microorganisms that thrive in cold environments and known as psychrophiles/psychrotrophs. Their ability to survive in the cold environments is based on the capacity to synthesize coldadapted enzymes, such as amylases, proteases, lipases, pectinases, cellulases etc., along with other specific characteristics. Enzymes are essential constituents of all forms of life on the Earth, including prokaryotes, fungi, plants and animals. Commercial applications of microbial enzyme are attractive due to the relative ease of largescale production as compared to enzymes from plants and animals. At present, only ~2% of the microorganisms on the Earth have been commercially exploited and amongst these there are only a few examples of psychrophiles and psychrotrophs.