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Bacillus anthracis is that the infective agent that causes fatal anthrax. Guizhou Province is Associate in Nursing previous foci of anthrax within the southwest of China. Human anthrax has conjointly been often times reportable in Guizhou in recent year. However, there's restricted dataon the genetic background of native B. anthracis isolates in Guizhou Province. Strain-specific detection of this microorganism mistreatment molecular approaches has increased our information ofmicroorganism biology. within the gift study, we tend to used Multiple Locus Variable variety cycleRepeats (VNTR) Analysis (MLVA) assay to research the genetic characteristics of B. anthracis strains isolated in Guizhou Province and their relationships to worldwide distributed isolates.