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Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules with nucleotides, proteins and alkaloids. The process of removing phosphate group is called dephosphorylation. Alkaline phosphatases are most effective in an alkaline environment. It is also called as basic phosphatase In Gram-negative bacteria, alkaline phosphatase is located in the periplasmic space external to the cell membrane and space is much more focus to environmental variation than the actual interior of the cell, bacterial alkaline phosphatase is comparatively resistant to inactivation, denaturation, and degradation and has a higher rate of activity.