A trial was conducted at Department of Horticulture, Junagadh Agricultural University, Junagadh to investigate the effect of organic, inorganic and biofertilizer on growth, yield and quality of gladiolus cv. Psittacinus hybrid. Twelve treatment combinations of different nutritional sources were comprised with three replications. Results due to various treatments found significant. Application of (T3) was recorded for minimum days to first spike emergence (72.10 days), maximum plant height (91.88 cm), maximum number of stems per plant (2.20) and fresh and dry weight of plant (161.36 gm and 46.37 gm, respectively).