Biological markers or biomarkers are the indicators of a particular biological state before the appearance of manifestations in form of a disease. A variety of blood biomarkers represent pathology of diabetes. Analysis of blood is an important technique for determining physiological and pathological condition. People suffering from Type 2 diabetes mellitus (T2DM) were part of the study. The people with bleeding disorders and those suffering from other diseases were not included in the study. There were more female (F) diabetic patients than male (M) patients. This case control observational study is an attempt to interpret data obtained from biochemical investigation of diagnostic biochemical parameters of complete blood count and lipid profile for their pathological implication and in prediction risk of Type 2 diabetes mellitus and related complications among the healthy subjects. Normality test was done to check for normal distribution of the data among females (1) and males (2) and the correlation test showed no significant relation of the of biochemical parameters with PBSS.