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As already published by Shintani et al, gas plasma sterilization is the alternative sterilization procedures to the existing sterilization procedures due to applicable to the several sensitive healthcare products and attainable both sterility assurance level (SAL) of 10-6 and material/functional compatibility, which authorities and GMP (good manufacturing practice) are required. In addition there was progress in the field of plasma-based sterilization, there are still some questions. The aim of this editorial is to mention briefly some of un-resolved sterilization mechanisms as well as to draw further perspectives of sterilization technique.