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Pretreatment technologies have been developed to increase the bioconversion rate of biomass into fermentable sugar. The objective of this research was to investigate extrusion with thermostable α-amylase injection at different melt temperatures 95, 115 and 135°C on functional properties, ethanol content and conversion (%) of corn starch extrudates. Saccharomyces cerevisae (ATCC 24858) was used for ethanol production.