Six Algerian honeys from different floral origins, were examined for potential antibacterial and antiradical activity. The Folin-Ciocalteu assay was used to measure total phenol content (TPC) and the 2,2-diphenyl-picrylhydrazyl (DPPH) assay was used to determine the scavenging activity of the honey samples. An agar well diffusion assay and spectrophotometric method were used to assess antibacterial activity against two Gram negative strains (Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC 50071).Total phenolic content varied from 63.93 to 95.36 mg/100 g honey as gallic acid equivalent.
Citation: Ahmed M, Djebli N, Aissat S, Khiati B, Ãœnal M, et al. (2012) Antiradical Activity and Total Phenolics of Algerian Honeys and Antibacterial Effect against Gram-Negative Bacteria. J Microb Biochem Technol 4: 152-156. doi: 10.4172/1948-5948.1000087