Urticadioica, stinging nettle, is a species of the Urticaceae family. U. dioica agglutinin (UDA) is a component of U. dioica, purified from its rhizomes. UDA which has lots of medicinal properties has been used as a folklore remedy since ancient times. In this study, we investigated the cytotoxic and apoptotic effects of one of a good representative ingredients of Urtica dioica agglutinin (UDA), at different concentrations (1, 5, 10, 20 μg/ml) on AGS cancer cell line by carrying out WST-1 (cell proliferation), LDH (cytotoxicity), xCELLigence (real-time cell counter) and TUNEL (apoptosis) assays, respectively. According to our results different concentrations of UDA have anti-proliferative and apoptotic effects on AGS cell line after 48 hours. The RTCAassay indicated that lectin exhibited cytotoxicity, with an IC50 value 4.96μg/ml. Therefore, our current information regarding UDA confirms that UDA may be used in cancer therapeutic strategies as a primary cure in the future.