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The effect of copper sulphate on liver, gills and kidney of fish Channa punctatus was observed as metabolic indicators. The fish were exposed to sub-lethal (0.36 mg l-1) concentrations for 15, 30 and 45 days. The observations revealed that in the presence of copper sulphate the metabolic enzymes aspirate amino transferases (ASAT) and alanine amino transferases (ALAT) can enhance their activity, while catalase activity significantly reduced in response to elevated level of superoxide production in exposed fish as compared to healthy subjects. The major effect of copper sulphate toxicity on exposed fish shows as reduced amount of protein content as compared to unexposed fish. However, ASAT level have a significant negative correlation between catalase activity (r = 0.833, t = 6.3872, p 0.05) and protein content (r = 0.8916, t = 8.3540, p 0.05) in the exposed organs. These findings suggest that the significant increase of transaminase activity while reduced amount of catalase and protein content might be the consequences of tissue damage in Channa Punctatus.
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