Recombinant Escherichia coli JM109 harbouring the polyhydroxyalkanoate (PHA) biosynthesis gene (phaCABco) of Comamonas sp. EB172, an acid tolerant microbe, was examined for the production of PHAs from various carbon sources. The study demonstrated that the recombinant E. coli JM109 had the potential to utilize both sugar- and acid-based carbon sources, for the biosynthesis of both poly(3-hydroxybutyrate) P(3HB) and poly(3- hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymers.
Citation: Yee LN, Mumtaz T, Mohammadi M, Phang LY, Ando Y, et al. (2012) Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172. J Microb Biochem Technol 4: 103-110. doi: 10.4172/1948-5948.1000079