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Hepatitis B virus is a human infectious disease universally caused by the hepatitis B virus. Its genome size is 3.215 kb. Immunoinformatics tools have been used to predict the epitopes from seven putative protein viz. polymerase, large-S- and middle –S- Protein, S and X- protein, Precore/Core Protein, Core and E- antigen. Total 50 epitopes were predicted for MHC class I and 55 epitopes for class II MHC molecules. These epitopes showed highest binding score at optimum threshold. Epitopes may use as an antigen for diagnosis and also might be helpful for designing peptide based subunit vaccine against Hepatitis B virus.
