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Evaluation of Dry Blood Spot (DBS) Stored at Different Temperature for Detection of HIV Antibodies

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Evaluation of Dry Blood Spot (DBS) Stored at Different Temperature for Detection of HIV Antibodies

Mustafa EM Yassin et al. evaluated the uses of dry blood spot stored at 37°C, 45°C, and 50°C for detection for HIV antibodies by using Rapid test. They have used 95 blood specimens, 50 specimens were collected from HIV positive and 45 collected from HIV non-reactive patient, Dry Blood Spots (DBS) were prepared by applying 50 μl of whole blood on Whatman 3 filter paper (Whatman International® Ltd.), specimens of all DBS were stored at 37°C, 45°C and 50°C. All DBS were tested with two rapid tests (ACON ® One Step Test Device) and (ACCURATE ® test device) and to evaluate the effect of storage temperature and time on sensitivity and specificity, DBS were tested after 2 weeks, 4 weeks and 8 weeks.

Citation: https://www.omicsonline.org/evaluation-of-dry-blood-spot-dbs-stored-at-different-temperature-for-detection-of-hiv-antibodies-by-using-different-rapid-tests-2161-1076.S12-002.php?aid=13263

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