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A cross-sectional study was conducted from November, 2013 to April, 2014 to assess the prevalence of bovine babesiosis in Teltele District, North West Borena Zone, Southern Ethiopia. A total of 384 blood samples were collected from ear vein of cattle from four peasant associations (PAs). In this study the overall prevalence of bovine babesiosis was found 16.9% (65/384) using microscopic examination of Geimsa stained blood smear.