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The glioblastoma cells were passaged continuously 76 times in vitro for more than 6 months without alternation of cell morphology. The purity is more than 98% after 5 times of passages. The glioblastoma cell line was established successfully in vitro. Observed by phase contrast microscopy (A) Cultured 24 hrs (200×); (B) Cultured 72 hrs (100×); (C) 2 times of passages (200×); (D) 70 times of passages (200×).
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