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After discovery of malaria parasites and their transmission by Anophelines, until recently, malaria was antroponotic disease caused by four Plasmodium species. These parasites were circulated among humans by different vectors around the world. There are more than 100 species of Plasmodium, which can infect many animal species such as reptiles, birds, and various mammals. Four species of Plasmodium have long been recognized to infect humans in nature. It is clear that epidemiologic pattern of malaria is going to be changed to both anthroponotic and zoonoses. They are including risk of the mix infection of anthroponotic and zoonoses parasites.