The microbial load of milk is a major factor in determining its quality, which is indicates the cleanliness of the milking utensils, condition of storage, manner of transport as well as the cleanliness of the udder of the individual animal. The aim of this study was to determine microbial quality of cow’s milk. The study was conducted in Dawa Chefa District, Oromia Zone of the Amhara National regional state. A total of 50 samples of raw cow's milk were collected at morning from hotels (27 samples), cooperative (3 samples) and household producers (20 samples). The farmers (producers) that involve in the study were selected based on potential of milk production, market orientation and willingness of households. Samples of raw morning milk were taken from each sampling point twice in a month. During collection, approximately 250 ml raw milk sample were taken aseptically from the owner’s container then placed into sterile glass bottles.