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The infections can be attributed to various microorganisms such as Staphylococcus aureus, E. coli, Bacillus cereus and so on which can be present on the surface of healthcare products as bioburden and can come into contact with human tissues. Several studies have demonstrated that some of these microorganisms are resistant to antibiotics such as MRSA, VRE and so on and have an impact on the patients; this indicates the need for improved sterilization procedures free from bioburden, airborne, falling microorganisms or adhesive microorganisms as far as possible. However, some studies have shown that significant quantities of residues composed of salts, proteins and organic matter are left on operational instruments even after complete cleaning and sterilization. These observations raise serious health concerns because residue pathogens can be transmitted to pathogen free patients as iatrogenic diseases. Most serious pathogens included are the abnormal prion proteins, the principal factors for the transmission of Creutzfeld Jakob Disease; this could potentially be transmitted with contaminated instruments during brain operation.
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