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Lactoferrin (LF) is known to show various useful biological features such as antimicrobial, antiviral, antitumor and anti-inflammatory effects. However, orally administered LF undergoes enzymatic degradation in the gastrointestinal tract, resulting in extensive decrease of such functions. Chitosan microparticles loaded with LF were newly produced in order to develop an oral delivery system to elevate intestinal or systemic immunity. A high LF content, sufficient LF release and the particle size of submicron to a few micrometer size will be better to achieve biological potential or intestinal localization and retention of LF.
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