alexa Cloning and its Applications

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Cloning and its Applications

In mammalian fertilization process, female pronucleus share male pronucleus to form the zygote which undergoes mitotic division yielding totipotent cells. After morula compaction, the embryonic cells differentiate into two types of cells; the inner cell mass cells which have the pluripotent stemness criteria to form the embryo proper, and the trophectoderm which would share the placenta formation. In vitro -without spermatozoa contribution and using somatic cells instead- the oocyte was found to has extraordinary astonishing capabilities to process the genetic materials of transferred somatic cells through different mechanisms; 1) Reprogramming the somatic cells even when it is enucleated, as in case of somatic cell nuclear transfer (cloning) which produces a copy of genetic materials of the somatic cell donor animal; 2) Enforcing the somatic cells nuclei to bypass an obligatory mitotic cell cycle checkpoints and undergo premature condensation; and 3) Enforcing the somatic cells for entry of meiosis through the reduction division as known by semi-cloning or haploidization. Moreover, it was found that the early meiotic ooplasm was able to induce initiation of a meiosis-like reducing division in mitotic nuclei originating from differentiated somatic cells. Thus, the meiotic machinery property of the ooplasm can be helpful for generation of artificial oocytes or male germ cells through injecting male and/or female somatic cells into enucleated mature or immature oocytes.

 

Citation: Saadeldin MI (2015) Cloning and its Applications. Clon Transgen 4:e118. doi: 10.4172/2168-9849.1000e118

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