The aim of biotechnology is to understand the biochemical, cellular, and functional aspect of all the proteins encoded in the genome. Molecular cloning or recombinant DNA technology (RDT) is an important practice in research labs that is used to create copies of a particular gene for downstream applications, such as sequencing, mutagenesis, genotyping or heterologous expression of a protein. Heterologous expression is used to produce large amounts of a protein of interest for functional and biochemical analyses. It is a traditional technique frequently used to know about biochemical and functional aspect which involves the transfer of a DNA fragment of interest from one organism to other via a self-replicating genetic material plasmid.
Kumar A (2015) Cloning: A Vital Tool for the Production Transgenics . Clon Transgen 4:e119. doi:0.4172/2168-9849.1000e119