Citalopram is one of a class of antidepressants known as selective serotonin reuptake inhibitors. Citalopram blocks the reuptake of serotonin at the serotonin reuptake pump of the neuronal membrane, enhancing the actions of serotonin on 5HT1A autoreceptors. SSRIs bind with significantly less affinity to acetylcholine, histamine and norepinephrine receptors than tricyclic antidepressant drugs Using UV spectrophotometer a simple, economical least time consuming and accurate method for comparison of different brand of citalopram, has been developed. The assay is based on the UV absorbance maxima at about wavelength of 244 nm using distilled water as solvent. Four different brand of citalopram was dissolved in buffer of pH 1, pH 4 and water and then various dilutions are prepared (200 ppm, 100 ppm, 50 ppm and 25 ppm). The absorbance of these drugs was measured at 244 nm against the solvent blank and the assay was calculated by using the absorbance of active. We obtain linear relationship of four brands of citalopram when diluted to form 200 ppm, 100 ppm, 50 ppm, 25 ppm and absorbance of all brand are maximum in pH 4 .Squared correlation coefficient value of all the brands of citalopram are well within the limit.