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The finding test the hypothetical statement of theory 1 of Boy’o universal theory of therapy (BUTT 1), it maintained that any process in body depends on STP and MCT interaction, signaling medium enumerated in most literature shed light on genesis of various processes in the body [1], be they binding or non binding actions, STP encompass complex interrelated series of communicating cascades of proteins and enzymes, each cascade have particular attributes which they utilized in processes of transduction, they transmit signals by assigning roles to each cascade components and in this regard, adapter, recruiter, amplifier, deactivators and other forms exist, so that by ‘on’ and ‘off’ mode, signal is amplify down the cascade stream, how these event are mediated by many interconnected pathway backbone is remains to be demystified completely, which is why I incorporated mathematical equations and calculation in this study, importantly, the calculation and novel equation serve effective tool which handles the complexity of STP and MCT, the organ bath/drug bioassay essentially ensured determination of the autonomic nature of the extract from contraction and relaxation response observed when extract and drugs interact with the receptors on the isolated rabbit ileum in organ bath, the autonomic nature of extract so determined furnished the result from which the mechanism of action of extract as it relate to signal transduction pathways {STPs} of seven of the isolated rabbit ileum were inferred in accordance to stipulation of previous literatures, for instance Ganong [2] show that certain autonomic action are mediated via muscarinic and adrenergic receptors as well as their respective STPs such as cAMIP3- PKA pathways. Qingzhen et al.