Ethanol fermentation with cellulase inhibitors

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Ethanol fermentation with cellulase inhibitors

The basal ethanol fermentation mixtures were composed of 50 g/L glucose, 5 g/L yeast extract, 5 g/L Bacto peptone, 2 g/L NH4Cl, 1 g/L KH2PO4 and 0.3 g/L MgSO47H2O in 20 mM citrate buffer (pH 5.0) and were inoculated 3 g/L (dry weight) S. cerevisiae BY4743. We also added cellulase inhibitors (standard hydrolysate: 7.6 g/L tannic acid, 1.2 g/L trimesic acid and 1.2 g/L Gallic acid or IEEH: 0.7 g/L tannic acid, 0.5 g/L trimesic acid and 0.06 g/L Gallic acid) to the basal ethanol fermentation mixture, which was fermented at 120 rpm for 72 hours at 30°C. The control was maintained as described above without the addition of any of the compounds.

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