Glutinone (1), coixol (2), friedelin (3), glutinol (4), and betulinic acid (5) were isolated from the plant Scoparia dulcis. Their structures were identified using mass and 1D- and 2D- NMR techniques. All the compounds were tested for their immunomodulatory potential in oxidative burst assay. Compound 1 showed a significant inhibitory effect on the release of reactive oxygen species (ROS) from zymosan activated cells from whole blood (IC50 = 4.3 ± 0.6 μg/mL) as well as from isolated PMNs (IC50 = 5.0 ± 0.3 μg/mL) as compared to standard drug ibuprofen in whole blood (IC50 = 11.2± 1.9 μg/ mL) and in isolated PMNs (IC50 = 2.5± 0.6 μg/mL) shows that it is more active in whole blood as compared with isolated PMNs. Compound 1 when further tested for its effect on pro-inflammatory cytokines TNF-α, IL-1β and on nitric oxide (NO), was found to moderately inhibiting the production of TNF-α (19%) at a concentration 25 μg/mL. On the other hand a weak inhibitory effect of this compound was also observed on the production of IL-1β and NO production, whereas, compounds 2-5 showed no effect (IC50 = >100) on the release of ROS from zymosan activated cells.
Citation: Sharma KR, Adhikari A, Jabeen A, Dastagir N, Kalauni SK, et al. (2015) Immunomodulatory Studies on Triterpenoids from Scoparia dulcis Linn Biochem Pharmacol (Los Angel) 4:182. doi:10.4172/2167-0501.1000182