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The aim of this study was to evaluate the effect of β-cyclodextrin (β-CD) on phospholipids and cholesterol in raw pasteurized milk with the purpuse on removal cholesterol from the milk fat. Total phospholipids decrease slightly in β-CD treated milk but were not signinificantly affected by the effect of the β-CD. β-CD is an effective oligosacharide for cholesterol removal from milk with more than 97% of reduction and does not affect significantly the individual phospholipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserina) of the milk fat globule membrane.