Fluorescence imaging in life sciences is a part of the daily routine in many laboratories. The properties of fluorescent dyes applied in experiments play a key role for the success of an experiment and quality of maintained data. We give an overview on emerging opportunities in using quantum dots for fluorescent imaging. Quantum dots are inorganic nano-sized semiconductors with highly interesting size-(and material/surface)- dependent optical properties such as very sharp emission bands. A comparison of applications of quantum dots (QDs) with conventional dyes suitable for conjugation with biomolecules like antibodies or oligonucleotides will reveal similarities and differences. We will further show how QDs can enhance scope and application of conventional dyes. Our focus lies in strategies for using quantum dots to increase either multiplexing capabilities in immunostaining or for applying them as effective partner for exciting other fluorophores in sensitive FRET-assays.
Citation: Ruppert C, Kohl M, Jacob LJ, Deigner HP (2015) Multiplexing in Bioassays. Biosens J 4:124.