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Retinoblastoma (RB) is an eye cancer found in children. Early diagnosis of RB is very crucial for the treatment to be effective. miRNA 18a is highly expressed in the serum of RB patients which can be used as a diagnostic marker to detect RB. A pillar based micro mixer is designed and fabricated on PMMA platform to hybridize the target miRNA 18a with its complementary quenched probe throughout the flow. The fluorescence can be measured in the monitoring well by the fluorescent reader at a particular wavelength and compared with the controls.
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