Metabolomics deals with analysis of highly complex biological samples resulting in insufficient separation of relevant metabolites from matrix substances with one-dimensional liquid chromatography. Comprehensive twodimensional liquid chromatography (2D-LC) is a promising technique for the analysis of complex samples because of its increased peak capacity. Comprehensive 2D-LC-MS/MS has been applied for a targeted metabolomics approach regarding metabolites from RNA catabolism. Combination of a Zorbax Eclipse Plus C18 column with a Zorbax Bonus-RP column resulted in increased peak capacity, minimisation of matrix effects and excellent reproducibility.
Willmann L, Schlimpert M, Pan D, Bauer C, Trafkowski J, et al. (2015) Comprehensive Two-Dimensional Liquid Chromatography in Metabolome Analysis. J Chromatogr Sep Tech 6:288. doi:10.4172/2157-7064.1000288