The present study aims at assessing Bisphenol A (BPA) level in water used in laboratories. A total of five types of water commonly used in laboratory (tap, softened, distilled, double distilled, commercial LC-MS pure water), were analyzed and BPA was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only the samples of ultrapure water showed BPA levels above detection limits (>0.004 ng/mL). The mean BPA level ranged from 0.008 to 0.473 ng/mL. A higher mean BPA level was found in water obtained from ultrafilter process compared to commercial sources. Activated carbon filtration is necessary to achieve a BPA free level in ultrapure water.