It is known that active oxygen plays an important role in a reproduction. However, no report has so far investigated the influence of active oxygen produced from gp91phox NADPH oxidase on newborns. In this study, we investigated the influence of active oxygen on the weight of newborns using graviditas gp91phox-knockout (gp91phox-/-) mice. Gestational C57BL/6j (control), gp91phox-/-, and insulin-like growth factor-1-knockout (IGF-1) mice were examined and the weight of the newborn mouse pups were analyzed.
Gp91phox-/-and IGF-1/- mouse pups had low weight compared with control mice. When the control mice were treated with an inhibitor of reactive oxygen species (ROS), the newborn weight decreased. Conversely, when the gp91phox-/- mice were treated with an activator of ROS, the newborn weight increased, however, it remained low in the IGF-1-/-mice.