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This study was carried out to investigate the spatial patterns of Hepatitis C and its prevalence in males and females in Ghadezai Tehsil District Buner Khyber Paktunkhawa. The patients’ data i.e. residential addresses were taken from the district head quarter Dagger Buner. This data was used for the analysis of the Hepatitis C disease. All the patients’ locations were marked correctly through GPS in the form of x y co-ordinates. These co-ordinates were used to performed spatial analysis, mapping and other quarries of this disease by using GIS software. The results show that the spatial pattern of Hepatitis C in Ghadezai Tehsil was random and uneven.