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DNA cloning have been permitted the development of modern biology, in particular in the last 40 years we have passed from theorize DNA cloning to clone a gene, using fast techniques and widespread in all research laboratories, moreover there are company that can clone any DNA fragment, coding or regulative at relatively low cost; these clones can be used to transform bacterial cells and transfecting eukaryotic cells. To understand how these techniques of DNA manipulation have become so easy to use, a brief history can be made to understand what were the most important discoveries in this field.
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