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Jatropha curcas L. a member of Euphorbiaceae family, is being promoted as a biofuel crop and has attracted great interest in the scientific community. The cultivation in wastelands of Jatropha curcas as a biofuel crop avoids the alleged food vs. fuel dilemma. The infestation of collar rot caused by fungus Macrophomina phaseolina imposes heavy yield loss in this plant. In this review we highlight the significance of a pathogenesis related gene, JcPR-10a from this biofuel crop towards stress/defence tolerance. The JcPR-10a recombinant protein exhibit RNase and DNase activity interestingly, the protein also possess antifungal activity against Macrophomina. The docking analysis reveals the binding of three BAP (6-benzylaminopurine) molecules at the active sites of JcPR-10a protein.